In‐situ Investigations on Gold Nanoparticles Stabilization Mechanisms in Biological Environments Containing HSA

نویسندگان

چکیده

Nanoparticles (NPs) developments advance innovative biomedical applications. However, complex interactions and the low colloidal stability of NPs in biological media restrict their widespread utilization. The influence properties on for gold with 5 40 nm diameter two surface modifications, methoxy-polyethylene glycol-sulfhydryl (PEG) citrate, NaCl human serum albumin (HSA) protein solution, is investigated. This study based small-angle X-ray scattering (SAXS) methods allowing in-situ monitoring physiological conditions. PEG coating provides high both sizes. For a stable 3D self-assembled body-centered cubic (BCC) arrangement detected an interparticle distance 20.7 ± 0.1 nm. In this increases to 21.9 by penetration inside ordered structure. citrate-capped NPs, different mechanism observed. particles attach surfaces, appropriate concentration proteins results suspension. Cryogenic transmission electron microscopy (Cryo-TEM), UV–visible spectroscopy, dynamic light (DLS) support SAXS results. findings will pave way design synthesize controlled behaviors

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ژورنال

عنوان ژورنال: Advanced Functional Materials

سال: 2021

ISSN: ['1616-301X', '1616-3028']

DOI: https://doi.org/10.1002/adfm.202110253